Objective To justify the symmetry of bilateral humeral heads through 3D CT imaging techniques in order to provide a reference for surgical treatment of shoulder injury. Methods 3D CT data of 164 pairs of humeral heads from healthy population were studied and were dissected,three-dimensionally reconstructed, packed,inversed,and aligned by VMedViewer softwear,and then imported into VRmesh software. The left-toright distance between corresponding points on the surface of bilateral humeral heads,the average point-topoint distance, maximal point-to-point distance,location of maximal point-to-point distance were recorded. Results The average point-to-point distance was 0.16 0.26 mm with the largest distance of 5.90mm. The average maximal point-to-point distance was 3.27 0.87mm. The distributions of maximal point-to-point distances in different parts of humeral head were as followed:11 cases in posterior greater tuberosity,65 cases in posterior articular surface,40 cases in intertubercular groove,13 cases in anterior greater tuberosity,19 cases in anterior articular surface,and 16 cases in lesser tuberosity. Conclusion The configuration of humeral head was very similar between both sides. The average point-to-point distance between corresponding points on both sides of the humeral head was 0.16 0.26mm.