finding optimal threshold for correction error reads in dna assembling

ISCAS OpenIR > 2009年期刊/会议论文

	finding optimal threshold for correction error reads in dna assembling
	Chin Francis Y. L.; Leung Henry C. M.; Li Wei-Lin; Yiu Siu-Ming
	2009
会议名称	9th Asia Pacific Bioinformatics Conference
页码	-
会议日期	JAN 13-16,
会议地点	Beijing, PEOPLES R CHINA
收录类别	sci,istp
出版地	CURRENT SCIENCE GROUP, MIDDLESEX HOUSE, 34-42 CLEVELAND ST, LONDON W1T 4LB, ENGLAND
出版者	BMC BIOINFORMATICS
ISSN	1471-2105
部门归属	Chin, Francis Y. L.; Leung, Henry C. M.; Yiu, Siu-Ming Univ Hong Kong, Dept Comp Sci, Pokfulam, Hong Kong, Peoples R China. Li, Wei-Lin Chinese Acad Sci, Inst software, State Key Lab Comp Sci, Beijing 100190, Peoples R China.
摘要	Background: DNA assembling is the problem of determining the nucleotide sequence of a genome from its substrings, called reads. In the experiments, there may be some errors on the reads which affect the performance of the DNA assembly algorithms. Existing algorithms, e. g. ECINDEL and SRCorr, correct the error reads by considering the number of times each length-k substring of the reads appear in the input. They treat those length-k substrings appear at least M times as correct substring and correct the error reads based on these substrings. However, since the threshold M is chosen without any solid theoretical analysis, these algorithms cannot guarantee their performances on error correction. Results: In this paper, we propose a method to calculate the probabilities of false positive and false negative when determining whether a length-k substring is correct using threshold M. Based on this optimal threshold M that minimizes the total errors ( false positives and false negatives). Experimental results on both real data and simulated data showed that our calculation is correct and we can reduce the total error substrings by 77.6% and 65.1% when compared to ECINDEL and SRCorr respectively. Conclusion: We introduced a method to calculate the probability of false positives and false negatives of the length-k substring using different thresholds. Based on this calculation, we found the optimal threshold to minimize the total error of false positive plus false negative.
语种	英语
WOS记录号	WOS:000265601900015
引用统计
内容类型	会议论文
URI标识	http://ir.iscas.ac.cn/handle/311060/8192
专题	2009年期刊/会议论文
推荐引用方式 GB/T 7714	Chin Francis Y. L.,Leung Henry C. M.,Li Wei-Lin,et al. finding optimal threshold for correction error reads in dna assembling[C]. CURRENT SCIENCE GROUP, MIDDLESEX HOUSE, 34-42 CLEVELAND ST, LONDON W1T 4LB, ENGLAND:BMC BIOINFORMATICS,2009:-.